The test should preferably be completed during the first cycle of urine in the morning. This is essential as the first cycle of urine in the morning contains more amount of “HCG”. HCG (Human Chorionic Gonadotropin hormone) is a pregnancy hormone, present in the urine of a pregnant woman and helps in detecting the pregnancy. The test can also be attempted at any other time during the day with fresh urine. However, this urine must be stored in the urinary bladder for about 4-5hrs. This helps in increasing the amount of HCG in the urine.